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. 2008 Feb 11;153(6):1281–1287. doi: 10.1038/bjp.2008.19

Figure 1.

Figure 1

Effects of simvastatin and combination with L-NAME or PTIO on platelet aggregation. (a) In the presence of L-arginine (100 μM), washed platelets were preincubated with DMSO or simvastatin (20–200 μM) for 5 min. Then, collagen (10 μg ml−1) or AA (100 μM) was added to trigger platelet aggregation. (b) In the presence of L-arginine, washed platelets were preincubated with simvastatin (20–80 μM) or L-NAME (100 μM) and simvastatin (20–80 μM) for 5 min. Then, collagen was added to trigger platelet aggregation. (c) In the presence of L-arginine, washed platelets were preincubated with simvastatin (60 or 80 μM) or simvastatin (60 or 80 μM)+PTIO (50 μM) for 5 min, followed by addition of collagen. *P<0.05, **P<0.01 and ***P<0.001, as compared with the respective simvastatin or collagen-treated platelets. Percent inhibition of platelet aggregation is presented as means±s.e.mean (n=6). AA, arachidonic acid; DMSO, dimethyl sulphoxide; L-NAME, NG-nitro-L-arginine methyl ester; PTIO, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide.