Effect of salmeterol on the induction by dexamethasone of p57KIP2, GILZ and MKP-1. BEAS-2B cells were treated with dexamethasone (1 μM) salmeterol (100 nM), a combination of the two drugs or vehicle for 1–18 h and harvested for real-time PCR analysis of p57KIP2, GILZ, MKP-1 and GAPDH mRNA using SYBR GreenER technology. Data are expressed as a ratio to the housekeeping gene, GAPDH. See text for further details. GILZ, glucocorticoid-inducible leucine zipper; MKP, mitogen-activated protein kinase phosphatase.