Figure 4.

2-Aminoethoxydiphenyl borate (2-APB)-induced inhibition of transient receptor potential melastatin 2 (TRPM2) currents by ADP-ribose (ADPR) was not related to the cytosolic ATP or Ca²⁺. (a and b) Representative traces of the 2-APB-induced inhibition of TRPM2 currents activated by ADPR (500 μM) in the presence (200 nM; a) and absence (b) of intracellular Ca²⁺. Whole-cell configuration was established at arrows. (c) Inhibitory effects of 2-APB (30 μM) on TRPM2 currents activated by ADPR (500 μM) in the presence (200 nM) and absence of intracellular Ca²⁺. Numbers in parentheses indicate the number of cells tested.