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. 2008 Mar 4;98(6):1068–1075. doi: 10.1038/sj.bjc.6604279

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Copyright © 2008 Cancer Research UK

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Differential expression of FOXO1 in drug sensitive and resistant ovarian cancer cell lines and ovarian cancer samples. (A) Comparative analysis of FOXO1, FOXO3a, FOXO4, total and phosphorylated Akt (pAkt) expression in KF28, KFr13 and KFr13Tx cells by western blot analysis. β-Actin served as a loading control. (B) RTQ–PCR analysis demonstrated significant higher FOXO1 mRNA levels in KFr13Tx cells when compared to KF28 cells. The results show mean±s.d. of triplicate measurements and ^**P<0.001. (C) The representative immunohistological staining of FOXO1 of a chemotherapy-responder and non-responder is shown. The adjacent table summarises the number of patients with immunohistochemical reactivity of FOXO1 according to the chemotherapeutic response to paclitaxel-based chemotherapy in ovarian cancer patients.