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dbcAMP and/or NGF up-regulate Trk A mRNA levels. (A) RT–PCR reaction products for Trk A detection under different experimental conditions. (B) dbcAMP and NGF each induced a twofold increase in Trk A mRNA levels (P < 0.05) with respect to control, whereas treatment with NGF + dbcAMP induced a sixfold increase with respect to control (n = 6; P < 0.05, Student’s t test). C, control; A, dbcAMP (5 mM); N, NGF (50 ng/ml); NA, NGF + dbcAMP; F, fibroblasts; P, PC12 cells.
