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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Biomaterials. 2008 Jan;29(1):75–84. doi: 10.1016/j.biomaterials.2007.09.014

Fig 4. Cell survival as a function of ECM and scaffold composition.

Fig 4

Scaffolds (4.2 mm diameter) composed of either 100 % microspheres (solid lines) or 50 % microspheres (dotted lines) and seeded with 1.5 million human GFP human myoblasts were implanted subcutaneously in the back of NOD/SCID mice. Cells were embedded in a fibrin ECM (open circles) or collagen ECM (filled circles). Datapoints represent GFP area as quantified by confocal analysis pre-implant and after 2 or 4 weeks in vivo. At 2 weeks in vivo, myofibers embedded in a collagen ECM on average covered more area than myofibers in the fibrin ECM; however this difference was not statistically significant. At 4 weeks, GFP area for all conditions was 10–17% (22±2% survival when area was normalized to preimplant GFP area), with no statistical differences for the tested conditions.