TABLE 3.
Labeling schemes used for structure and orientation samples
|
Distances (assuming an isolated spin pair)
|
|||||
|---|---|---|---|---|---|
| Name | Labeling scheme | Off | Bound, lyophilized | Bound, hydrated | |
| (Structure, 13C-15N) | |||||
| LRAP-L42A46 | LRAP-PELPLEAWPATDKTKREEVD | 5.3 ± 0.5 | 5.5 ± 0.5 | 5.6 ± 0.5 | Random coil |
| LRAP-A49T53 | LRAP-PELPLEAWPATDKTKREEVD | 5.9 ± 0.5 | 6.1 ± 0.5 | 6.9 ± 1.0 | Random coil |
| (Orientation, 13C-31P or 15N-31P) | |||||
| LRAP-A46* | LRAP-PELPLEAWPATDKTKREEVD | >12 ± 1.0 | 7.0 ± 0.5 | 8.0 ± 0.5 | Next to HAP |
| LRAP-A49 | LRAP-PELPLEAWPATDKTKREEVD | >12 ± 1.0 | 6.0 ± 0.5 | — | Next to HAP |
| LRAP-K52 | LRAP-PELPLEAWPATDKTKREEVD | >10 ± 1.0 | 5.8 ± 0.5 | 4 ± 1.0 | Next to HAP |
Residues in bold indicate isotopic labels. For the structure samples, bold residues represent backbone 13C′, bold underlined residues represent backbone 15N. For the orientation samples, bold italic residues are 13Cβ (A46), 13Cα (A49), or 15Nζ (K52).
*
Labeling scheme reported previously (41).