Effect of MBCD treatment on total hydrolysis by sPLA2. The total amount of hydrolysis was evaluated for the experiments of Fig. 2 at 500 s (represented by ADIFAB GP) as explained in Materials and Methods. Data were analyzed by two-way analysis of variance to calculate the contribution of ionomycin (1.4% of variation, p = 0.09), MBCD (37% of variation, p < 0.0001), and interaction between the two (0% of variation, p = 0.95), n = 32–35.