TABLE 2.
Parameter | Literature (plit) | Estimated (p0) |
---|---|---|
V+,PA, min−1 | 8.2 × 10−3 | |
Vcell,CDS, min−1† | 9.6 × 10−3 | |
Vcell,PGPS, min−1† | 4.7 × 10−3 | |
Vcell,PGPP, min−1 | * | 3.2 |
Vcell,PAP, min−1 | 4.1 × 10−2 | 7.7 × 10−2 |
Vcell,MGS, min−1 | 1.8 × 10−3 | 1.4 × 10−2 |
Vcell,DGS, min−1 | 3.5 × 10−4 | 1.2 × 10−3 |
Vcell,GPDGS, min−1 | * | 1.5 × 10−2 |
CDS KM,PA, mol % | * | 9.3 × 10−2 |
PGPS KM,CDPDAG, mol % | * | 1.0 × 10−2 |
PGPP KM,PGP, mol % | * | 38.2 |
PAP KM,PGP, mol % | 10 | |
MGS KM,DAG, mol % | 6 | 5.2 |
DGS KM,MGlcDAG, mol % | 1 | |
GPDGS KM,PG, mol % | * | 25 |
GPDGS KM,DGlcDAG, mol % | * | 85 |
CDS KM,CTP, mM† | 0.58 | |
PGPS KM,G3P, mM† | 0.32 | |
MGS KM,UDP-Glc, mM | 0.4 | |
DGS KM,UDP-Glc, mM | 0.14 | |
CDS Keqm | 0.22 | |
CTP, mM | 0.5 | |
UDP-Glc, mM† | 0.4 | 2.5 |
PPi, mM† | 1 | |
G3P, mM† | 0.2 | 0.32 |
Parameters are obtained through a multiobjective optimization using the evolutionary algorithm SRES (55). Parameters marked * have not been measured. Parameters marked † correspond to E. coli and are given less weight in the optimization. See Appendix B for references and details of the literature parameters.