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. 1998 Jun 9;95(12):7109–7114. doi: 10.1073/pnas.95.12.7109

Figure 1.

Figure 1

Hydrophobicity plot of SEL-12 and schematic representation of SEL-12 constructs. (A) Hydrophobicity plot of SEL-12 constructed by using the Kyte–Doolittle algorithm (25) with a window size of 15. HRs are numbered as in ref. 13. (B) SEL-12 constructs used in the LacZ fusion approach and the rescue assay. SEL-12ΔHR6–8∷LacZ, LacZ placed after the HR9 of SEL-12 with the region of HR6 through HR8 deleted; SEL-12ΔHR6–8∷TM∷LacZ, a construct similar to SEL-12ΔHR6–8∷LacZ but including a synthetic transmembrane domain (21) at the fusion junction (amino acid P410 of SEL-12) between sel-12 and lacZ cDNAs.