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. 1998 Jun 9;95(12):7109–7114. doi: 10.1073/pnas.95.12.7109

Figure 4.

Figure 4

Hydrophobicity plot of human PS1 and schematic representations of PS1∷LacZ and PS1∷TM∷LacZ constructs. (A) Hydrophobicity plot of PS1, using a window size of 15 (ref. 25). The hydrophobic regions are numbered as in ref. 13. (B) PS1 constructs used in the LacZ fusion approach for topological studies. PS1HR7∷LacZ, LacZ placed after the HR7 of PS1; PS1HR7∷TM∷LacZ, a fusion similar to PS1HR7∷LacZ with a synthetic transmembrane domain at the fusion junction (amino acid L377 of PS1); PS1HR9∷LacZ, LacZ placed after the HR9 of PS1; PS1HR9∷TM∷LacZ, a fusion similar to PS1HR9∷LacZ with a synthetic transmembrane at the fusion junction (amino acid P429 of PS1); PS1ΔHR8∷LacZ, LacZ placed after the HR9 of PS1 with the HR8 deleted; PS1ΔHR8∷TM∷LacZ, a fusion similar to PS1ΔHR8∷LacZ with a synthetic transmembrane domain at the fusion junction (amino acid P429 of PS1).