Table 2.

Two-sample binomial tests for GOSlim assignments of paralogous family and singleton proteins in Arabidopsis

GOSlim assignmenta	Singletons (%)	Paralogous genes (%)	P-valued
Hydrolase activityb	7.5	12.6	<1e-5
Kinase activityc	10.4	5.5	<1e-5
Nucleotide bindingc	10.2	4.6	<1e-5
Protein binding, otherc	12.9	8.2	<1e-5
Transcription factor activityb	4.2	9.0	<1e-5
Receptor activityc	1.9	0.7	<1e-5
DNA bindingb	4.1	7.2	<1e-5
Oxygen bindingb	0.1	1.4	<1e-5
Receptor bindingc	0.5	0.1	<1e-5
Carbohydrate bindingc	0.7	0.3	<1e-3
Lipid bindingb	0.3	0.8	0.001
Structural molecule activityb	1.6	2.5	0.002
Enzyme regulator activityb	0.7	1.4	0.005
Molecular function, otherb	1.8	2.5	0.011
Transporter activityb	5.0	6.0	0.019
Nucleic acid binding, otherc	2.6	2.0	0.027
Motor activityb	0.2	0.5	0.03
Transferase activity	5.3	6.1	0.053
RNA binding	1.5	1.9	0.099
Binding, other	12.3	11.3	0.102
Signal transducer activity	1.0	0.8	0.132
Catalytic activity, other	12.4	11.7	0.244
Transcription regulator activity	1.3	1.5	0.743
Chromatin binding	0.2	0.1	0.803
Translation factor activity, nucleic acid binding	0.6	0.6	1
Nuclease activity	0.7	0.8	1

^a GoSlim assignment classifications were performed as described in the Materials and Methods.

^b Enrichment of GOSlim annotations in paralogous protein families compared to singletons.

^c Reduction of GOSlim annotations in paralogous protein families compared to singletons.

^d Benjamini and Hochberg correction for multiple testing.