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Culture supernatant proteins from extracellular protein secretion system mutants. Cells were grown in Y-mannitol medium for 4 days. Proteins were precipitated with trichloroacetic acid. A, R. l. bv. viciae 3841 wild type; B, toaD mutant; C, tobD mutant; D, prsD mutant; E, virB6 mutant; F, autA mutant; G, autB mutant; H, autC mutant. Protein bands missing in the lane representing the prsD mutant are marked (*).
