Table 2.
Culture supernatant proteins from R. l. bv. viciae 3841 were identified by MALDI-ToF MS.
| Band | Apparent weight [kDa] | Top MOWSE score | Number of matched fragments | Predicted protein | Theoretical weight [kDa] | Predicted function |
| *1 | 89.1 | 205 | 18 | RL2961 | 95.0. | cadherin-like protein |
| *2 | 59.2 | 165 | 15 | pRL90140 | 79.0. | parallel beta-helix repeat protein |
| *3 | 57.7 | 113 | 15 | pRL100309 | 60.7 | cadherin-like protein |
| *4 | 52.2 | 78 | 8 | RL2412 | 64.6 | glycosyl hydrolase |
| 5 | 50.2 | 243 | 20 | RL4651 | 63.7 | dipeptide-binding protein |
| 6a | 46.5 | 55 | 7 | RL0778 | 58.8 | dipeptide-binding protein |
| *6b | 46.5 | 195 | 16 | RL3024 | 51.6 | PlyB |
| 7 | 41.5 | 101 | 10 | RL0728 | 44.4 | flagellar hook |
| 8 | 38.5 | 174 | 14 | RL4218 | 47.6 | sorbitol-binding protein |
| 9 | 37.5 | 76 | 8 | RL3329 | 43.4 | membrane bound lytic tranglycosylase |
| 10 | 35.6 | 77 | 7 | RL3745 | 38.9 | Leu/Ile/Val-binding protein (BraC) |
| 11a | 34.1 | 60 | 6 | RL0720 | 33.6 | flagellin |
| 11b | 34.1 | 96 | 9 | RL2375 | 38.9 | basic membrane lipoprotein |
| *12a | 29.8 | 58 | 5 | pRL100451 | 24.9 | RapA2 |
| 12b | 29.8 | 60 | 6 | RL0196 | 35.0. | basic membrane lipoprotein |
| 12c | 29.8 | 44 (53) | 5 | RL0489 | 35.4 | sugar-binding protein |
| 12d | 29.8 | 36 (51) | 4 | RL0718 | 31.0. | flagellin |
| 13 | 27.7 | 129 | 10 | RL0518 | 39.4 | ribose-binding protein |
| 14 | 25.0. | 113 | 9 | RL2753 | 27.9 | arginine/ornithine-binding protein |
| 15 | 20.4 | 57 | 5 | RL1369 | 24.5 | pentapeptide repeat protein |
| 16 | 17.1 | 85 | 6 | RL2404 | 20.5 | peptidyl prolyl cis-trans isomerase |
| *17 | 16.3 | 83 | 6 | RL1580 | 15.3 | nucleoside diphosphate kinase |
| 18 | 16.0 | 70 | 5 | RL2697 | 19.5 | COG3184 |
The protein bands labelled in Fig. 6. were cut out from the gel and digested with trypsin. The masses of the tryptic digest were identified by MALDI-ToF MS and searched against a R. l. bv. viciae 3841 genome database. Band #, protein band as labelled in Fig. 6., Band from which more than one protein was identified are indicated (e.g. 6a, 6b). Apparent weight, molecular weight of the proteins in the given band as estimated from the electrophoretic mobility. Top MOWSE score, statistical score calculated by the MASCOT program for the R. l. bv. viciae 3841 indicated in column 'predicted protein', scores above 50 indicate >95% confidence. Number of matched fragments, number of fragment masses which could be assigned to the predicted protein. Predicted protein, proteins scoring more than 50 as MOWSE score. Theoretical weight, calculated molecular weight of the indicated proteins. Scores obtained after refining search parameters are shown in brackets. An asterisk indicates PrsDE-dependence.