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. Author manuscript; available in PMC: 2008 Mar 27.
Published in final edited form as: Mol Cell. 2007 Dec 28;28(6):1045–1057. doi: 10.1016/j.molcel.2007.12.005

Figure 4. MDC1 domains required for IRIF formation by 53BP1 and BRCA1.

Figure 4

(A). IRIF of 53BP1 and BRCA1 in MDC1+/+ MEF cells or MDC1−/− MEF cells. IR doses were as shown.

(B). IRIF of 53BP1 and BRCA1 in MDC1−/− MEF cells stably expressing myc-tagged mouse wtMDC1 or mutant MDC1 alleles as indicated. Cells received 3Gy of IR.

(C). Statistical evaluation of the experiments shown in (A and B). Nuclei containing more than five 53BP1 foci or ten BRCA1 foci were scored as positive for IRIF. >150 cells were analyzed for each MDC1 allele indicated. Paired t-test for both 53BP1 foci and BRCA1 foci between MDC1+/+ cells and MDC1−/− cells: P < 0.00014%; between “MSIP” and “MDC1”, “ΔFHA”, or “ΔPST”: P < 0.01%; between “MSIP” and “ΔSQ” or between “MDC1” and “ΔFHA” or “ΔPST”: NS.

(D). BRCA1 and 53BP1 IRIF in 53BP1+/+ or 53BP1-/- mouse EC cells. Cells received 3Gy of IR.

(E). 53BP1 and γ-H2AX IRIF in BRCA1 mutant HCC1937 cells (IR doses as shown).