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. Author manuscript; available in PMC: 2008 Mar 27.
Published in final edited form as: Mol Cell. 2007 Dec 28;28(6):1045–1057. doi: 10.1016/j.molcel.2007.12.005

Figure 6. “HR suppression” function of 53BP1 does not require H2AX.

Figure 6

(A). I-SceI-induced GFP+ frequencies in HCC1937 HR/SCR reporter cells (p53−/−BRCA1 mutant), transiently transfected with expression plasmids as indicated. Bars represent mean of triplicate samples. Error bars indicate SEM. Paired t-test between “p53” and “Vector”: NS; between “F-53BP1” and “Vector”: P < 1.2%; between “F-53BP1” and “F-D1521R”: P < 0.04%. Levels of transiently transfected proteins are shown under the chart.

(B). I-SceI induced GFP+ frequencies in H2AX+/+ and H2AX−/− ES HR/SCR reporter cells transiently transfected with HA-F-53BP1 or HA-F-D1521R expression plasmids. Bars represent mean of triplicate samples. Error bars indicate SEM. Paired t-test between “F-53BP1” and “F-D1521R”: P < 0.3% in H2AX+/+, and P < 1.6% in H2AX−/− cells. Levels of HA-F-53BP1 and HA-F-D1521R proteins are shown under the chart.

(C). I-SceI induced GFP+ frequencies in four additional H2AX−/− ES HR reporter clones transiently transfected with HA-F-53BP1 or HA-F-D1521R expression plasmids. Each clone containing either the HR reporter or the HR/SCR reporter is indicated. Bars represent mean of triplicate samples. Error bars indicate SEM. Paired t-test between “F-53BP1” and “F-D1521R”: P < 1.55% in HR#8, P < 1.20% in HR#28, P < 1.62% in SCR#13, and P < 0.50% in SCR#43.