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. Author manuscript; available in PMC: 2009 Mar 10.
Published in final edited form as: Exp Cell Res. 2008 Mar 10;314(5):1177–1191. doi: 10.1016/j.yexcr.2007.12.009

Figure 4.

Figure 4

Transfection with siRNA against Krp1 sequences specifically reduces Krp1 expression. (A) Krp1 protein is comprised of an N-terminal BTB-BACK domain and a C-terminal kelch repeat region. In the nucleotide sequence, Krp1 siRNA 1 targets an area within the kelch domain, while Krp1 siRNA 2 targets a sequence between the BTB-BACK and the kelch domains. (B) Transcript levels were analyzed by real time PCR 48 hours after transfection with control or Krp1 siRNA and normalized to levels in mock-transfected controls. Krp1 transcript levels were significantly decreased within 48 hours of transfection with either Krp1 siRNA 1 or 2, while control siRNA had no effect. Reduction of Krp1 expression was specific, as transcript levels of other genes were unchanged. p-values compared to control siRNA-transfected samples: *p<0.05; **p<0.01. Data are the means and SEM of three independent experiments. (C) Immunoblot analysis of specific proteins at the indicated times after mock (m) or Krp1 siRNA (si) transfection. Krp1 protein levels are significantly reduced by day three after transfection, whereas muscle myosin heavy chain and sarcomeric actin levels are unchanged compared to mock-transfected controls. Densitometric analysis of Krp1 protein levels relative to mock-transfected controls is shown on the right; data are the means and SEM from three independent experiments.