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. Author manuscript; available in PMC: 2009 Feb 28.
Published in final edited form as: Gene. 2008 Jan 28;410(1):122–128. doi: 10.1016/j.gene.2007.12.005

Figure 1. 1% Agarose Gel Electrophoresis of Degenerative RT-PCR and PCR-RACE products.

Figure 1

The reverse transcriptase-polymerase chain reaction (RT-PCR and Rapid amplification of cDNA ends (RACE) methods were employed to synthesize the cDNA fragments as described in section 2.2. A) Degenerative RT-PCR product. A 342nt fragment was obtained. Lane 1: Marker, Lane 2: RT-PCR product. B) 5′-RACE Product. A 318 nt fragment was obtained. Lane 1 Marker, Lane 2 5′-RACE Product (C) 3′-RACE products. Two DNA fragments of 640 nt and 455nt were obtained. Lane 1 Marker, Lane 2 3′-RACE Product