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. Author manuscript; available in PMC: 2009 Feb 28.
Published in final edited form as: Gene. 2008 Jan 28;410(1):122–128. doi: 10.1016/j.gene.2007.12.005

Figure 5. Northern Blot Analysis.

Figure 5

(A) Denaturing gel electrophoresis of total L. pectinata RNA. Lane 1: RNA ladder, Lane 2: 5 μg total RNA, Lane 3: 10 μg total RNA and Lane 4: 20 μg total RNA. B. HbIII Northern Blot analysis. The membrane was hybridized with a 32P- labeled 342 nt HbIII cDNA RT-PCR fragment. The estimated size of the HbIII mRNA was 1.1Kb. Increasing concentration of total RNA were used; Lane 1: 5 μg, Lane 2: 10 μg and Lane 3: 20 μg. C. HbII Northern Blot analysis. The same membrane was re-hybridized with a 32P labeled HbII cDNA RT-PCR fragment as a control for specific hybridization. The expected 2.1 Kb band was shown for HbII. Increasing concentration of total RNA were used; Lane 1: 5 μg, Lane 2: 10 μg and Lane 3: 20 μg.