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. Author manuscript; available in PMC: 2009 Feb 13.

Published in final edited form as: Mol Cell Endocrinol. 2007 Dec 8;283(1-2):114–126. doi: 10.1016/j.mce.2007.11.031

Fig. 2 — Response of receptor induction properties of GREtkLUC with different concentrations of GMEB-2. Triplicate wells of 1470.2 cells were transfected with 100 ng GREtkLUC and 20 ng Renilla null luciferase reporters to examine the induction properties of endogenous GR (A), and 30 ng of co-transfected hPR-B cDNA plasmid (B and C), in the presence of different concentrations of GMEB-2 plasmid: 10 ng in B or 100 ng in A and C. The responses after treatment with agonist (Dex (A) or R5020 (B, C)), or antagonist (Dex-Ox (A) or Dex-Mes (B, C)) were determined and plotted as in Fig. 1. Similar results were obtained in four (A) and three (B, C) additional experiments.

Fig. 2 — Response of receptor induction properties of GREtkLUC with different concentrations of GMEB-2. Triplicate wells of 1470.2 cells were transfected with 100 ng GREtkLUC and 20 ng Renilla null luciferase reporters to examine the induction properties of endogenous GR (A), and 30 ng of co-transfected hPR-B cDNA plasmid (B and C), in the presence of different concentrations of GMEB-2 plasmid: 10 ng in B or 100 ng in A and C. The responses after treatment with agonist (Dex (A) or R5020 (B, C)), or antagonist (Dex-Ox (A) or Dex-Mes (B, C)) were determined and plotted as in Fig. 1. Similar results were obtained in four (A) and three (B, C) additional experiments.