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Effect of Ubc9 on PR and GR transactivation properties in CV-1 cells. Triplicate wells of CV-1 cells were transfected with 100 ng GREtkLUC and 20 ng Renilla null luciferase reporters plus 0.03 ng PR-B plasmid, 0.03 ng PR/GR plasmid, or 100 ng GR plasmid ± 150 ng Ubc9 plasmid (total plasmid for GR experiments = 403 ng). The cells were induced and analyzed, and the results plotted, as in Fig. 1. Similar results were obtained in 2-4 additional experiments.
