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. Author manuscript; available in PMC: 2009 Feb 13.
Published in final edited form as: Mol Cell Endocrinol. 2007 Dec 8;283(1-2):114–126. doi: 10.1016/j.mce.2007.11.031

Fig. 5.

Fig. 5

Regions of STAMP that associate with PR and GR in two-hybrid assays. (A) Cartoons show regions of STAMP that are retained in each of the constructs used below. Plasmid names are that of STAMP followed by the sequence retained, with the letters N and C designating the N- and C-terminal residues of the receptor respectively. Abbreviations of STAMP domains: TTL = tubulin tyrosine ligase, CID = coactivator interaction domain, RIDs = receptor interaction domains, which is comprised of three subdomains I-III (He and Simons; Jr., 2007). (B&C) Cos-7 cells were transiently transfected with 86 ng VP16/GR ± 1 μM Dex (B) or 1 ng VP16/PR-B ± 20 nM R5020 (C) plus GAL fusions of STAMP segments (molar equivalent to 80 ng of GAL/STAMP834C) and 100 ng of FRLuc reporter. The average relative luciferase activities (n = 2-6 for B; = 3-7 for C) were determined as in Fig. 1 and plotted. The values in parentheses above the bars indicate the fold increased interaction ± 1 μM Dex (B) or 20 nM R5020 (C).