Figure 5. A possible position of EpsH at the tip of the T2SS pseudopilus.

The conserved hydrophobic crevice of Vibrio cholerae EpsH facing outwards when EpsH is placed at the tip of a tentative pseudopilus model (for details see Materials and Methods). The model T2SS pseudopilus was constructed from EpsG homology-modeled subunits, based on the K. oxytoca GspG structure¹⁵, that are superimposed onto PilE subunits within the assembled PilE pilus⁴⁹. The modeled EpsG subunits are colored dark green. The helix of PilE is colored light green. EpsH superimposed onto the tip of the PilE pilus is colored red. The 18 residues that form the hydrophobic crevice on the surface of EpsH are colored yellow.