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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Methods. 2008 Feb;44(2):74–80. doi: 10.1016/j.ymeth.2007.08.001

Figure 1.

Figure 1

[3’-32P] labeling tRNA by nucleotide exchange. (A) Two step reaction scheme for nucleotide exchange by tRNA nucleotidyltransferase. (B) PEI cellulose TLC plate showing [α-32P] ATP before (left) and after (right) incorporation into tRNA with a yield of 76%. (C) 10% denaturing polyacrylamide gel showing 20 μM tRNAfMet after treatment different concentrations of tRNA nucleotidyltransferase: Lane 1: 6 μM; Lane 2: 2 μM; Lane 3: 0.67 μM; Lane 4: 0.22 μM.