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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Methods. 2008 Feb;44(2):74–80. doi: 10.1016/j.ymeth.2007.08.001

Figure 2.

Figure 2

Aminoacylation analysis of [3’-32P] tRNAAla by AlaRS. S1 nuclease digestion of Ala-tRNAAla results in free [32P] AMP and [32P] Ala-AMP that can be separated on a PEI cellulose TLC plate in glacial acetic acid/1 M NH4Cl/water (5:10:85). Reaction yields as a function of AlaRS concentration: Lane 1: 100 pM; Lane 2: 1 nM; Lane 3: 10 nM; Lane 4: 100 nM, Lane 5: 1 μM.