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Developmental Immunology logoLink to Developmental Immunology
. 1996;5(1):17–24. doi: 10.1155/1996/14738

In Vitro and In Situ Characterization of Fish Thymic Nurse Cells

Emilio Flaño 1,, Francisco Álvarez 1, Pilar López-Fierro 1, Blanca E Razquin 1, Alberto J Villena 1, Agustín G Zapata 2
PMCID: PMC2275971  PMID: 8828008

Abstract

We present an enzyme- and immuno-cytochemical, and ultrastructural characterization of trout thymic nurse cells (TNCs). Our data suggest that isolated trout thymic multicellular complexes are epithelial cells with acidic compartments that may be involved in the processing of antigens and in the generation of the MHC-II proteins that these cell express, and also that isolated TNCs are the In Vitro equivalent of the pale and intermediate electronlucent epithelial cells located in the inner zone of the trout thymus, constituting indirect evidence of the phylogenetical relationships of the inner zone of the teleost thymus with the thymic cortex of higher vertebrates.

Keywords: Thymic epithelium, thymic nurse cells, trout thymus

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