Figure 5.
The temporal expression of viral proteins by vVACV-C7L and vMYXV-M64R in VERO and HeLa cells. VERO (A) and HeLa cells (B) were infected at a MOI of 10 PFU per cell with vVACV-C7L or vMYXV-M64R. At the indicated time after infection (hpi), the level of C7L or M64R proteins was determined by Western blot with an anti-V5 antibody. The levels of VACV proteins E3L, WR148 and host cell protein Hsp70 were also determined by Western blot as respective control for VACV early, late protein expression and gel loading.