Skip to main content
. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Virology. 2007 Dec 3;372(2):372–383. doi: 10.1016/j.virol.2007.10.023

Figure 6.

Figure 6

The defect of the host-range restricted viruses at suppressing cellular PKR and eIF2-α phosphorylation in HeLa cells correlates with a defect of the viruses at maintaining E3L expression. (A). The same cell lysates as described in Fig 5B were used to determine the levels of total and phosphorylated PKR and eIF2-α proteins in HeLa cells that were infected with vVACV-C7L or vMYXV-M64R for various time. The level of phosphorylated PKR and eIF2-α proteins were determined by Western blot with mAbs against phospho-PKR (Thr446) and phospho-eIF2α (Ser51). The blot was then stripped, and the levels of total PKR and eIF2-α were determined by Western blot with a mAb against total PKR and a polyclonal antibody against total eIF2-α. (B). HeLa cells were infected with the indicated viruses for 4 h and the level of total and phosphorylated PKR and VACV E3L proteins were determined by Western blot. (C). The same samples as shown in B were analyzed by Western blot with a multiplex of antibodies, including antibodies against phospho-p90RSK (Ser-308), phospho-Akt (Ser-473), phospho-p44/42 mitogen-activated protein kinase (Thr-202/Tyr-204), phospho-S6 ribosomal protein (Ser-235/236), and total eIF4E.

HHS Vulnerability Disclosure