Figure 3.

Gel-blot analysis of transcripts from five cold-regulated genes in wild-type (WT) and esk1 Arabidopsis. Plants were grown at 22°C under 150 μmol quanta m⁻²⋅s⁻¹. Total RNA (10 μg) isolated from leaf tissue of nonacclimated plants (NA) or from plants cold acclimated at 4°C for 2 days (CA) was separated on a 1.2% agarose-formaldehyde gel and probed successively with cDNAs corresponding to the cold-regulated genes indicated. The ribosomal 25S rRNA was visualized on the nylon membrane by using ethidium bromide to demonstrate equal loading. Quantitative comparisons reported in the text are based on PhosphorImager analyses of the blots. The experiment was repeated four times with similar results.