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. 2008 Mar 17;9:7. doi: 10.1186/1471-2091-9-7

Figure 1.

Figure 1

Fiber formation of ORE chimeras is faster than that with the wild type ORD of PrP. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Graphs are plotted as %RFU (Relative fluorescence units) versus time. (A) Sup35NM unseeded (solid line) and Sup35NM seeded with sonicated preformed fibers (dotted line). Reaction was seeded with 2.5% (w/w) of preformed sonicated fibers. (B) Unseeded reactions Sup35NM (solid line), SP5NM (dotted line), SP8NM (short dashed line), SP11NM (dotted and dashed line) and SP14NM (long dashed line).