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. 2008 Apr 9;3(4):e1918. doi: 10.1371/journal.pone.0001918

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) The top panel shows a WB of 293T cell extract transfected with or without p300 plasmid analyzed using an anti-acetyl lysine antibody; bottom panel shows the same membrane stripped and analyzed with an anti-WRN antibody. These cell extracts were used in the measurement of WRN exonuclease activities (B). (B) Lysates from 293T cells with (lane 3) or without (lanes 1 and 2) transfection with p300 expression plasmid were immunoprecipitated with antibodies against immunoglobulin G (IgG) (lane 1) or WRN (lanes 2 and 3). The washed immunocomplexes were incubated with the exonuclease substrate (top, 0.5 nM, final concentration) as described.