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. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Virology. 2007 Sep 27;370(1):151–157. doi: 10.1016/j.virol.2007.08.025

Fig. 2.

Fig. 2

Genome organization of the wild-type and mutant FV Pol open reading frames. The pol gene of SFVmac is shown in the upper panel with the relative location of the RT active center and the four amino acid residues associated with AZT resistance. The SFVmac mutants representing all possible combinations of the four mutations are shown in the lower panel together with the approximate levels of AZT resistance deduced from Table 2. The plasmids pBK04-ITTK and pBK64-ITTK are identical. Plasmid pBK04-ITTK was made by exchanging a pol gene fragment containing the four mutations and amplified from DNA of cell cultures infected with SFVAZTres for a corresponding fragment of pSK29-KISE and pBK64-ITTK was made by in vitro mutagenesis. M108 is a PFV mutant with the changed residues found in SFVAZTres.