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. 2008 Apr 9;3(4):e1961. doi: 10.1371/journal.pone.0001961

Figure 1. Distribution of mTOR immunoreactivity in peripheral sensory nerve fibers in the skin.

Figure 1

Confocal images of 40 µM thick frozen sections cut perpendicular to the skin surface of the rat hindpaw. A, B, Colocalization of phospho-mTOR (green) and nerve fibers marker PGP (red), a general marker of nerve fibers, in footpad of glabrous skin. Arrows indicate PGP- positive fibers not double-labelled. C, D, Colocalization of phospho-mTOR (green) and myelinated fiber marker N52 (red) in footpad of glabrous skin (C) and hairy skin (D). Arrows in C indicate N52- positive fibers not double-labelled. Asterisks (*) indicate double-labelled fibers in the area of Meissner corpuscles (sensory receptors within dermal papillae). E, Colocalization of phospho-mTOR and N52 in the dermis of the glabrous skin. In A, B and E the single staining for each antibody and the merged image are shown from left to right. In A-E double staining appears in yellow. mc, Meissner corpuscles; ep, epidermis; d, dermis; f, follicle. A, E, single focal planes; B-D, merge of 22–24 z-focal planes (20–21 µm depth). Scale bars, A-E: 50 µm.