FIGURE 1.

The C terminus of GEF contains the DNA-binding domain. A, a schematic representation of GST-GEF fusion proteins utilized in EMSA analysis. Residue numbers are shown for full-length (FL) and truncated versions of GEF. B, eluted GST-GEF fusion proteins were analyzed by EMSA to detect binding to the Domain I oligonucleotide. Increasing amounts of fusion proteins were mixed with radiolabeled Domain I oligonucleotides, separated over a non-denaturing polyacrylamide, and analyzed by autoradiography.