FIGURE 2.

Homo-oligomerization of GEF. GST-GEF can homo-oligomerize, but not within the DNA-binding domain (DB). A, schematic representation of GST- and HA-tagged GEF utilized in co-precipitations. GEF-11 comprises residues 68–387 of GEF-FL. B, nuclear extracts from COS-7 cells transiently expressing HA-GEF-11 were incubated with various GST-GEF fusion proteins bound to glutathione-Sepharose beads. Protein complexes were washed, eluted by denaturation, and analyzed by SDS-PAGE and immunoblotting for HA. FL, full-length.