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. 2008 Mar 21;283(12):7429–7437. doi: 10.1074/jbc.M800481200

FIGURE 3.

FIGURE 3.

Biotinylated domain I oligonucleotide can specifically precipitate GEF. A, schematic of the human GLUT4 promoter and the biotinylated oligonucleotides designed as described under “Experimental Procedures.” B, nuclear extracts from COS-7 cells transiently transfected with empty vector (mock) or a vector expressing GEF (GEF) were incubated with biotinylated oligonucleotides corresponding to Domain I (D) or a mutant Domain I unable to bind GEF (Dmut). DNA-oligonucleotide complexes were precipitated with streptavidin-agarose, eluted by denaturation, and analyzed by SDS-PAGE and immunoblot for GEF and compared with 10% of input lysates (lanes 5 and 6).