FIGURE 9.

HDAC5 specifically associates with the mouse Glut4 promoter in a differentiation-dependent manner. A, chromatin immunoprecipitation assays were performed using HDAC5 antibody, RNA polymerase III (Pol III), or non-immune IgG antibody. Lane 1 shows the PCR performed on the input DNA not subjected to immunoprecipitation. Lane 7 shows the PCR performed with water. Lane 5 is the positive control for the PCR using immunoprecipitation with RNA polymerase III and a positive control primer set for mouse. B, nuclear extracts and cytoplasmic extracts were prepared from 3T3-L1 adipocytes harvested at days 2, 4, and 6 post-differentiation. The nuclear extracts were blotted for HDAC5, and cytoplasmic extracts blotted for GLUT4. Blots shown are representative of three independent experiments. C, nuclear extracts from adipocytes harvested at days 0, 2, 4, and 6 post-differentiation were blotted for MEF2A, MEF2D, and GEF. Results shown are representative of two independent experiments.