Absorbance and fluorescence emission spectra of in vitro bilin
addition reactions with aporHT-AP (HT-ApcA/ApcB). A, absorbance
spectra of the control reaction (solid line), the reaction containing
CpcS-I (medium dashes), HT-CpcU (short dashes), or both
CpcSU (long dash triple dots). B, shows the room
temperature, fluorescence emission spectra of the same reactions upon
excitation at 590 nm with 10-nm slit widths. The maxima for some absorbance or
fluorescence emission of some of the products are indicated.