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EerI does not disrupt the interaction between p97 and atx3. Detergent extracts of 293T cells treated with Me2SO (DMSO) or EerI were subjected to immunoprecipitation with either control (IgG) or anti-p97 (αp97) antibodies. The immunoprecipitated material was immunoblotted (IB) with the indicated antibodies. Where indicated, a fraction of the extract (input, 15%) was directly analyzed by immunoblotting.
