Table 5. .
Probes Used for EMSA DNA Binding Assays
| Class of Binding Sited |
||||||
| Gene or Transcript |
Probe Sequencea | Positionb (bp) | Boundc | FOXP2 | FOXP | FOX |
| Consensus | agcttTATTTATgttgttttgtat | … | No | − | − | − |
| PM5-1 | ctttaagAATTTGTgtaagc | −643 | Yes | + | − | − |
| PM5-2 | gacgggagATTTTGTtgtg | −360 | No | + | − | − |
| CALCRL-1 | cacagATTTGTtagattttttttc | −436 | No | + | − | − |
| CALCRL-2 | aataTATTTATtctaagtag | 232 | Yes | − | + | − |
| SYK-1 | caaagccTTTTGTaataattaaag | −565 | Yes | + | − | − |
| SYK-2 | ggtttAATTTATTTGgttgtgg | −616 | No | ++e | − | − |
| MAPK8IP1-1 | gcctcccAATTTCaggtgag | 255 | No | + | − | − |
| MAPK8IP1-2 | tgcTATTGTCccatttcacag | −499 | Yes | − | − | + |
| CER1 | gaaggatgttAATTTTtttg | −603 | Yes | + | − | − |
| CER1M | gaaggatgttAACCCTtttg | … | No | − | − | − |
| SLC17A3-1 | gaataTAGTTATctCTTTGTcc | −626 | Yes | + | + | − |
| SLC17A3-1M | gaataTAGGGATctCGGGGTcc | … | No | − | − | − |
| SLC17A3-2 | ccATTTCTtaaccTATGTATgat | −602 | No | + | + | − |
| SLC17A3-3 | ttttgtatAATTTGagaa | −489 | Yes | + | − | − |
| PSEN2-1 | gggcgTTTTGTtcttcttctc | −193 | No | + | − | − |
| PSEN2-2 | ccccTGTTTATTGCcttaataag | 224 | Yes | − | − | ++e |
| LNPEP-1 | tctgtgttTATTTATggtctg | −416 | Yes | − | + | − |
| LNPEP-2 | ATTTATggtcTGTTTCTggaatg | −407 | No | + | − | + |
| COX11-1 | cgggtaAGTTTGcgtt | −136 | No | + | − | − |
| COX11-2 | tcgcgagATTTGAcctctcg | −10 | No | + | − | − |
| NFK | ccgggggtgatttcactccccg | … | No | − | − | − |
a
Core binding sites are represented in capital letters. Bases in bold font indicate deviation from or mutation of the consensus binding site.
b
Position relative to transcriptional start site.
c
Ability to be bound by FOXP2 during EMSA.
d
A plus sign (+) indicates the presence of the consensus binding site, and a minus sign (−) indicates the absence of the site.
e
Indicates two overlapping sites.