Figure 5. .

FOXP2 overexpression confirming functional regulation of targets by FOXP2. A, Cell lysate from SH-SY5Y cells transfected with empty vector or FOXP2 isoform I, run on an SDS-PAGE gel and transferred to PVDF membrane. The membrane was hybridized with anti-FLAG antibody and anti-GAPDH antibody. B, Nineteen genes tested by qRT-PCR. The average of seven replicates and SEMs are indicated. Genes with a down-regulation in expression are shown in red, whereas those with a positive change in gene expression are shown in blue. Genes with significant difference in expression between control and FOXP2-overexpressing cells are indicated with an asterisk (*) (P<.05, by Student’s t test).