Figure 5. .

Hierarchical clustering of methylation data from cerebral cortex, cerebellum, and pons samples analyzed in experiments 2 and 3. Columns represent samples, rows correspond to CpG sites, and heat maps showing relative methylation differences (red indicates more methylated; blue indicates less methylated) from a handful of loci analyzed are represented below the clustering dendrograms. All clustering analyses were performed using uncentered correlation and pairwise average linkage. A, Methylation profiles of 1,505 CpG sites from 33 cerebra samples (16 from normal individuals, 13 from individuals given diagnoses of autism, and 4 from individuals with bipolar disorder) were clustered. Clustering does not reveal disease-specific branches. B, Methylation profiles of 1,505 CpG sites from 26 cerebella samples (13 from normal individuals and 13 from individuals given diagnoses of autism) were clustered. Clustering does not reveal an autism-specific branch. C, Methylation profiles of 1,505 CpG sites from seven pons samples (three from normal individuals and four from individuals with bipolar disorder) were clustered. Clustering does not reveal a bipolar-specific branch.