Table 6. .

Quantitative Real-Time PCR Results of Five Genes Assayed in Paired Cerebellum and Cerebral Cortex Samples from 15 Individuals^[Note]

Measure	RASSF1	HDAC7A	GABRB3	EN2	HTR2A
Methylationa	CM > CBL	CBL > CM	CBL > CM	CBL > CM	CM > CBL
Distanceb	200	200	440	6500	1400
Fold changec	−2.0	1.5	1.4	−100	40.4
P	.011	.007	.210	<.001	<.001
Expressiond	CBL > CM	CM > CBL	CM > CBL	CBL > CM	CM > CBL

Note.— CBL = cerebellum; CM = cerebral cortex.

^aMethylation level from greatest to least, based on β value.

^bDistance (in bp) to transcriptional start site from the locus showing differential methylation across brain regions.

^cFold change = 2^−ΔΔCT; ΔΔC_T = (C_{T cerebrum target gene}−C_{T phosphglycerate kinase 1})−(C_{T cerebellum target gene}−C_{T phosphglycerate kinase 1}).

^dQuantitative real-time PCR result. For each gene, brain-region expression is ranked from greatest to least.