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. 2008 Apr 4;283(14):9475–9487. doi: 10.1074/jbc.M709587200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Assays of cofactor activity for factor I-catalyzed cleavage of C3b. Inferred identities of Coomassie-stained protein bands on SDS-PAGE gels are indicated. MWM, molecular weight markers. A, results of an end point C3b cofactor assay (excess C3b and FI in the absence (lane 2) and presence of 1 μm FH1-2 (lane 4), 1 μm FH2-3 (lane 5), and 1 μm FH1-3 (lane 6). Molecular weight markers are in lane 1. B, C3b cofactor assay with excess FI and C3b in the presence of FH at 0, 0.84, 2.53, 7.6 nm (lanes 1-4) or FH1-3 at 0.84, 2.53, 7.6, 22.8, 68.4, and 205 nm (lanes 5-10).