TABLE 5.

Inhibition of mouse liver BHMT and BHMT-2 activities (relative) by methyl donor substrates and products

Assays (500 μl) contained 2.5 mm dl-Hcy and 250 μm methyl donor (0.5 μCi). BHMT-2 assays were done in the presence of 62.5 μm CBHcy.

Ligand	[Ligand]	BHMT	BHMT-2
	μm
Enzyme alone	100	100
Bet	0.25		102
	2.5		96
SMM	0.25	97
	2.5	96
AdoMet	0.25	97	97
	2.5	95	85
DMSA	0.25	71	99
	2.5	18	94
DMSP	0.25	97	98
	2.5	89	83
DMG	0.25	36	97
	2.5	5	81
Met	0.25	98	91
	2.5	62	52