FIGURE 3.

FOXO3A increases peroxiredoxin III promoter activity in human cardiac fibroblasts. Human cardiac fibroblasts were co-transfected at 40% confluence with a reporter plasmid containing the DNA-binding elements of FOXO3A in the Prx III promoter (pGL3-Prx III FDBE) and a plasmid containing human FOXO3A (pcDNA3-FLAG-FOXO3A). Alternatively, cells were transfected with a reporter plasmid containing the full-length Prx III promoter (pGL3-Prx III promoter) with or without pcDNA3-FLAG-FOXO3A for 48 h. To control for transfection efficiency, all cells were also co-transfected in 1:10 ratio with Renilla luciferase plasmid. Cells were serum-deprived for 24 h and harvested for luciferase assays as described under “Experimental Procedures.” The data represent mean ± S.E. of luciferase relative luminescence units (RLU) from four different experiments normalized to corresponding Renilla luciferase activity. *, **, p < 0.005 versus control.