FIGURE 2.

UV-visible absorption spectra of purified CYP170A1 in Tris-HCl buffer (50 mm, pH 8.2). A, oxidized absolute spectrum in solid line; reduced spectrum using Na₂S₂O₄ in dashed line. B, reduced CO difference spectrum showing unique 440 nm compared with the normal reduced CO difference spectrum at 450 nm of CYP105D5. C, type I binding spectrum resulting from addition of epi-isozizaene (0–30 μm) to CYP170A1 (2.5 μm). The substrate epi-isozizaene structure is shown.