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. 2008 Mar 28;283(13):8202–8210. doi: 10.1074/jbc.M708944200

FIGURE 4.

FIGURE 4.

A, GITR stimulation decreased phosphorylation of Stat5 in IL-15-treated NK cells. Purified NK cells were cultured in the presence/absence of 10 ng/ml IL-15 in plates coated with the control IgG or anti-GITR antibody for 10, 20, and 30 min. Cells were collected and processed for Western blot analysis using anti-p-Stat5 and anti-p-p38 antibodies. B, GITR stimulation decreased phosphorylation of Akt and PDK1 in IL-15-treated NK cells. Purified NK cells were cultured in the presence/absence of 10 ng/ml IL-15 in plates coated with control IgG or anti-GITR antibody for 1, 2, or 3 days. Cells were collected and processed for Western blot analysis using anti-p-Akt, anti-Akt, or anti-p-PDK1 antibodies.