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. 2008 Mar 28;283(13):8266–8273. doi: 10.1074/jbc.M703097200

FIGURE 1.

FIGURE 1.

TcPUF6 is present in the ribosome-free fractions of sucrose gradients. a, the positions of the 40 and 60 S subunits, the 80 S ribosome monomer, and polysomes are indicated in the sucrose density gradient. b–d, Western blots of the collected fractions (30 μl) probed with anti-TcPUF6 (1/500) (b), anti-TcTif34 (1/500) (c), and anti-PEPCK (1/250) sera (d). Transfected parasites overexpress a TcPUF6-tagged protein. e, Western blot analysis of protein extracts from epimastigotes (lane 1), epimastigotes transfected with the pTEX-TAPTAG-transfected clones (lane 2), and the PUF6 A- and (4) B-transfected clones (lane 3), with antiserum against TcPUF6 protein (1/500 dilution). f, protein extracts were also tested with an anti-CBP serum (1/1000) (Upstate). g, the same blot was re-probed with an anti-PEPCK serum (1/500). h, immunolocalization of T. cruzi TcPUF6 in TcPUF6-TAPTAG-transfected epimastigote forms with anti-CBP antiserum (1/100). Bars, 5 μm.