TABLE 1.
Putative TcPUF6 targets identified by affinity purification assays are down-regulated in the transfected parasites and up-regulated in the metacyclic forms
| GeneID TSKa | Gene annotation | Immunoprecipitation assaysb | Fold change in transfectantsc | Fold change in metacyclicsd |
|---|---|---|---|---|
| Tc00.1047053508039.70 | Hypothetical protein | +,+,- | -4.9 | 0.34 ± 0.02 |
| Tc00.1047053506753.110 | Hypothetical protein (cytochrome b5-like) | -,-,- | -3.5 | NDe |
| Tc00.1047053504013.110 | Serine/threonine-protein phosphatase 2A | +,+,+ | -3.6 | 8.67 ± 1.02 |
| Tc00.1047053511523.20 | Hypothetical protein | +,+,- | -3.6 | 6.57 ± 0.53 |
| Tc00.1047053507641.80 | XRND1 exonuclease | +,+,- | -4.0 | 4.23 ± 0.35 |
| Tc00.1047053509179.50 | Hypothetical protein | +,+,- | -3.4 | 70.3 ± 6.48 |
| AAS47054 | PAT6 amino acid transporter | +,+,+ | -3.0 | 1.54 ± 0.14 |
| Tc00.1047053511215.119 | 69 kDa paraflagellar rod protein putative | +,+,+ | -8.4 | 7.81 ± 0.68 |
| EH035829 | Hypothetical protein | ND | -4.7 | ND |
a
Gene code from Trypanosoma cruzi Sequencing Consortium (when this is not available, the GenBank™ sequence ID is provided instead).
b
Presence (+) or absence (-) of the affinity purified transcripts in the three independent immunoprecipitation assays.
c
Fold change value of decreased expression determined by microarray analysis.
d
Fold change value of transcript expression levels in metacyclic trypomastigotes compared with epimastigote forms by quantitative PCR assays.
e
ND, not determined.